Vitamin D 25-OH total | IntroLab Belgrade

Vitamin D 25-OH total

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2500.00 rsd

Reverse time

1 day

Sampling

Drawing blood

Category

Immunohemistry

Written by:

Dr. Igor Švonja, MD

Updated:

January 21, 2026

Published:

May 19, 2023

Reading Time:

4 min

The total vitamin D analysis, conducted via a blood test, is essential for making a differential diagnosis for conditions such as rickets and osteomalacia. The blood test for vitamin D is used not only to assess the total vitamin D reserves in the body and identify possible hypovitaminosis or hypervitaminosis, but also to monitor the effectiveness of therapy that includes vitamin D supplementation.

Our specialized blood analysis laboratory, IntroLab, conducts blood draws, and our skilled technicians ensure a safe and efficient sample collection process.

Vitamin D – Details

Vitamin D is a liposoluble steroidal compound. There are two basic forms of vitamin D: vitamin D3, or cholecalciferol, which is produced in our skin, and vitamin D2, or ergocalciferol, which we cannot produce ourselves but intake through food or as a supplement.

In the human body, both forms of vitamin D undergo transformations. They are first converted into 25OH vitamin D, and then into 1,25OH vitamin D. 25OH vitamin D is the main form of vitamin D in our blood, but it is biologically inactive. From this form, the active hormone 1,25-dihydroxyvitamin D is produced. Due to its long half-life and higher concentration in the blood, the level of 25OH D is usually measured to assess and monitor a person’s vitamin D status.

Vitamin D plays a crucial role in the health of our bodies. It is essential for the proper growth and development of bones and other tissues. It also participates in the regulation of calcium, phosphate, and, to a lesser extent, magnesium metabolism. Additionally, vitamin D is important in regulating our immune system.

Why is a vitamin D blood test done?

A blood test for vitamin D is recommended for individuals with elevated or decreased levels of calcium, phosphate, and/or parathyroid hormone (PTH). It is also recommended for those with symptoms of bone disease. The test is indicated for individuals at risk of vitamin D deficiency, such as those on long-term anticonvulsant therapy or those suspected of having a vitamin D deficiency. Periodic testing is also important to assess the response to therapy in individuals diagnosed with a vitamin D deficiency.

Vrsta uzorka i vreme kada se uzima uzorak

Sample: Serum (venous blood)

Reverse time: 1 day

Preparation: No special preparation is needed. If you are using biotin as part of your therapy, at least 8 hours should pass from the time you last took biotin before you undergo the vitamin D level test.

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